CELL PROLIFERATION OF EPITHELIAL CELLS IN NORMAL HUMAN BREAST TISSUE DURING ARTIFICIAL MENSTRUAL CYCLES REGULATED BY ORAL CONTRACEPTIVES BY THE EXPRESSION OF PROLIFERATING CELL NUCLEAR ANTIGEN
Keywords:
Normal human breast, Oral contraceptives, Proliferating Cell Nuclear Antigen (PCNA)Abstract
The oral contraceptive effect on normal breast still remains unclear. The few reports
related to cell kinetics on the normal breast epithelium during the use of oral contraceptives
(OCs) are in disagreement and were made without discriminating the sort of OCs.
For those reasons and by the fact that they have been widely used, we propose to
determine the proliferative activity evaluated by the PCNA expression in epithelial cells
of normal breast tissue after the administration of levonorgestrel 0.15 mg and ethinyl
stradiol 0.03 mg during to cycles of 21 days (with seven days between pill packs).
Normal breast tissue was collected at the time of benign lump biopsy by removing
fragments of breast parenchima located at least 1 cm from the lesion. The biopsy took
place between the 22"d and 25,h day of menstrual cycle, and was determined on the basis
of the date of the menstruation and of serum progesterone measurement at the moment
of biopsy.
The material was fixed in 10% formalin for less than 12 hours and processed by
immonohistochemistry (PC-10). In the study group the average PCNA expression was
16.78% where as in the control group was 13.72% (Mann Whitney test p = 0.4173).
Our results showed that there is no difference in PCNA expression among OCs
users during two cycles of treatment.
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Copyright (c) 2026 Débora Garcia Y. Narvaiza, Afonso Celso Pinto Nazário, Vânia Nosé Alberti, Edmund Chada Baracat, Geraldo Rodrigues de Lima
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