UM NOVO MARCADOR DE ATIVIDADE PROLIFERATIVA (PCNA/CICLINA)
Keywords:
Câncer de mama, Fatores prognósticos, PCNAAbstract
Os autores relatam a importância dos fatores prognósticos no tratamento do câncer de
mama. Observam que os dados clínicos e os parâmetros anátomo-patológicos são insuficientes
para estimar o prognóstico, particularmente quando os linfonodos axilares apresentam-se
livres de comprometimento neoplásico. Evidenciam que nos recentes anos as pesquisas buscam
espelhar a agressividade tumoral através das informações da fração de crescimento tumoral e
das células em proliferação. Fazem uma revisão dos marcadores de atividade proliferativa e
apresentam um novo polipeptídeo, identificável em material parafinado, facilmente adaptável
à rotina laboratorial e que apresenta resultados altamente significativos quando correlacionados
com os fatores prognósticos.
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References
1. AALTOMAA S, LIPPONEN P. PAPINAHO S.
SYRJANEN K. Proliferating-cell nuclear antigen
(PC10) immunolabelling and other proliferation indices
as prognostic factors in breast cancer. J Cancer Rev
Clin Oncol 1993; 119: 288-294.
2. BRAVO R, CELIS E J. A search for differential
polypeptide synthesis throughout the cell cycle of Hela
cells. J Cell Biol 1980; 84: 795-802.
3. BRAVO R, FEY J S, BELLATIN J, LARSEN M P,
AREVALO J, CELIS E J. Identification of a nuclear
and of a cytoplasmic polypeptide whose relative
proportions are sensitive to changes in the rate of cell
proliferation. Exp Cell Res 1981; 136: 311-319.
4. BRAVO R, FRANK R, BLUNDELL APP,
MACDONALD-BRAVO H. Cyclin/PCNA is the
auxiliary protein of DNA polymerase 8. Nature 1987;
326: 515-517.
5. CARTER C L, ALLEN C. HENSON D E. Relation of
tumor size, lymph node status, and survival in 24.740
breast cancer cases. Cancer 1989; 63: 181-187.
6. CARVALHO F M. Análise de variáveis histológicas e
imunohistoquímicas na previsão da recidiva de tumores
phyllodes da mama. São Paulo, 1992 (Tese de
doutorado - Faculdade de Medicina da Universidade
de São Paulo).
7. CELIS E J, CELIS A. Cell cycle-dependent variations in the
distribution of the nuclear protein cyclin proliferating cell
nuclear antigen in cultured cells: Subdivision of S phase.
Proc Natl Acad Sci 1985; 82: 3262-3266.
8. CUMMINGS C M, FURNIVAL M C, PARSONS GP.
TOWNSEND E. PCNA immunostaining in breast
cancer. Aust NZ J Surg 1993; 63: 630-606.
9. FARO JUNIOR M P. Expressão imunohistoquímica do
PCNA (proliferating cell nuclear antigen) em sarcomas
de partes moles. São Paulo, 1993 (Tese de doutorado -
Faculdade de Medicina da Universidade de São Paulo).
10. GELB В А, КАMEL W O, LEBRUN P D, WARNKE A
R. Estimation of tumor growth fractions in archival
formalinfixed, paraffin-embedded tissues using two
anti PCNA/cyclin monoclonal antibodies. Am J Pathol
1992; 141: 1453-1458.
11. GHALI V S, LIAU S, TEPLITZ C, PRUDENTE R. A
comparative study of DNA ploidy in 115 fresh-frozen
breast carcinomas by image analysis versus flow
cytometry. Cancer 1992; 70: 2668-2672.
12.HALL A P, LEVISON A D. Review: Assessment of cell
proliferation in histological material. J Clin Pathol
1990; 43: 184-192.
13. HALL A P, LEVISON A D, WOODS L A, YU W C С,
KELLOCK B D, WATKINS A J et al. Proliferating
cell nuclear antigen (PCNA) immunolocalization in
paraffin sections: An index of cell proliferation with
evidence of deregulated expression in some neoplasms.
J Pathol 1990; 162: 285-294.
14. KEMP C. Valor prognóstico do antígeno nuclear de
proliferação celular (PCNA/Ciclina) nas pacientes com
câncer de mama no estádio II (T, N0,1 MO). São Paulo,
1995 (Tese de doutorado - Escola Paulista de Medicina
da Universidade Federal de São Paulo).
15.KURKI P, OGATA K, TAN M E. Monoclonal antibodies
to proliferating cell nuclear antigen (PCNA/Cyclin) as
probes for proliferating cells by immunofluorescence
microscopy and flow cytometry. J immunol Methods
1988; 109: 49-59.
16.LE DOUSSAL V, TUBIANA-HULIN M, FRIEDMAN
S, HACENE K, SPYRATOS F, BRUNET M.
Prognostic value of histologic grade nuclearcomponents of Scarff-Bloow-Richardson (S.B.R.).
Cancer 1989; 64: 1914-1921.
17.MARCHETTI E, QUERZOLI P, FERRETI S, FABRIS
G, NEUCI I. Assessment of proliferative rate of breast
cancer by Ki67 monoclonal antibody. Mod Pathol
1990; 3: 31-35.
18. MIYACHI K, FRITZLER M J, TAN E M. Autoantibody
to a nuclear antigen in proliferating cells. J immunol
1978; 121: 2228-2233.
19.NARITA T, FÜNAHASHI H, SATOH Y, TAKAGI H.
Proliferations cell nuclear antigen immunostaining in
breast cancer and its relation to prognosis. Jpm J Clin
Oncol 1993; 23(1): 20-25.
20. NISHIDA C, REINHARD P, LINN S. DNA repair
synthesis in human fibroblasts requires DNA
polymerase 8. J biol chem 1988; 263: 501-510.
21.OGATA K, KURKI P, CELIS E J, NAKAMURA M R,
TAN M E. Monoclonal antibodies to a nuclear protein
(PCNA/cyclin) associated with DNA replication. Exp
Cell Res 1987; 168: 475-486.
22. QUINN M C, WRIGHT A N. The clinical assessment of
proliferation and growth in human tumors: Evaluation
of methods and applications as prognostic variables. J
Pathol 1990; 160: 93-102.
23. SHRESTHA P, YAMADA K, WADA T, MAEDA S,
WATATANI M, YASUTOMI M et al. Proliferating cell
nuclear antigen in breast lesions: Correlation of c-erb
B-2 oncoprotein and EGF receptor and its
clinicopathological significance in breast cancer,
Virchows Archiv A Pathol Anat 1992; 421: 193-202.
24. ТАНАN R S, NEUBERG S D, DIEFFENBACH A,
YACOUB L. Prediction of early relapse and
shortened survival in patients with breast cancer by
proliferating cell nuclear antigen score. Cancer 1993;
71: 3552-3559.
25. TAKASAKI Y, DENG S J, TAN ME. A nuclear antigen
associated with cell proliferation and blast
transformation. J Exp Med 1981; 154: 1899-1909.
26. TAN K C, CASTILHO C, SO G.A, DOWNEY M K. An
auxiliary protein for DNA polymerase 8 from fetal calf
thymus. J biol chem 1986; 261: 12310-12316.
27.TOSCHI L & BRAVO R. Changes in cyclin/
proliferating cell nuclear antigen distribution
during DNA repair synthesis. J Cell Biol 1988;
107: 1623-1628.
28.TUBIANA M. Review article. The kinetics of tumour cell
proliferation and radiotherapy. Br J Radiol 1971; 44:
325-347.
29.TUBIANA M, PEJOVIC U H, CHAVANDRA N,
COUTESSO G, MALAISE E P. The long term
prognostic significance of the thymidine labeling index
in breast cancer. Int J Cancer 1984; 33: 441-445.
30. WASEEM H N & LANE P D. Monocional antibody
analysis of the proliferating cell nuclear antigen
(PCNA). J Cell Sci 1990; 96: 121-129.
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Copyright (c) 2026 Claudio Kemp, Geraldo Rodrigues de Lima, Vania Alberti, Filomena Marina Carvalho
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