MIR-26A AND MIR-181C PROFILE HIGHLIGHT AS POTENTIAL PROGNOSIS BIOMARKERS IN TRIPLENEGATIVE BREAST CANCER PATIENTS

Authors

  • Bárbara Danielle Silva Siqueira Fundação Ezequiel Dias, Diretoria de Pesquisa e Desenvolvimento, Serviço de Biologia Celular – Belo Horizonte (MG), Brazil.
  • Bianca Nataliene Carvalho de Camargos Fundação Ezequiel Dias, Diretoria de Pesquisa e Desenvolvimento, Serviço de Biologia Celular – Belo Horizonte (MG), Brazil.
  • Ana Luiza de Freitas Instituto Mário Penna, Laboratório de Pesquisa Translacional em Oncologia, Núcleo de Ensino e Pesquisa – Belo Horizonte (MG), Brazil.
  • Paulo Guilherme de Oliveira Salles Instituto Mário Penna, Laboratório de Pesquisa Translacional em Oncologia, Núcleo de Ensino e Pesquisa – Belo Horizonte (MG), Brazil.
  • Clécio Ênio Murta de Lucena Universidade Federal de Minas Gerais, Faculdade de Medicina, Departamento de Ginecologia e Obstetrícia – Belo Horizonte (MG), Brazil.
  • Letícia da Conceição Braga Instituto Mário Penna, Laboratório de Pesquisa Translacional em Oncologia, Núcleo de Ensino e Pesquisa – Belo Horizonte (MG), Brazil.
  • Luciana Maria Silva Lopes Fundação Ezequiel Dias, Diretoria de Pesquisa e Desenvolvimento, Serviço de Biologia Celular – Belo Horizonte (MG), Brazil.

DOI:

https://doi.org/10.29289/259453942022V32S2020

Keywords:

Breast cancer, Biomarkers, MicroRNA, Triple-negative breast cancer

Abstract

Objective: This retrospective cohort study aims to investigate the relative expression profiles of microRNAs (miR 26a,
125b, 181a, 181c, and 340-5p) in patients with triple-negative breast cancer (TNBC) and their relationship with clinical outcome. Methods: We included 10 patients with TNBC, treated at the Mário Penna Institute, Brazil, and 5 patients
without TNBC evidence, considered as control. This study was approved by the research ethics committee (CAAE protocol:
39741820.4.0000.9507). The total RNA extraction was performed from the formalin-fixed, paraffin-embedded (FFPE) tissues using the All Prep FFPE (Qiagen™). The RNA concentration was evaluated by the GE NanoVue Plus Spectrophotometer
and complementary DNA (cDNA) for each target was synthesized, as appropriate. To analyze the transcripts, the TaqMan
real-time PCR technique was used. The small nucleolar RNA RNU6-6P was used as an endogenous control. Changes in
miRNA expression were measured by method 2(-ΔΔCq). Results: The expression profile of microRNAs showed a great
variability among the TNBC patients, who reinforces the intratumoral heterogeneity of TNBC patients. One of 10 patients
showed overexpression of all miRNA evaluated, while 2/10 had underexpression from all of them. An underexpressed profile of miR 181c and 26a was seen in those samples that had a tumor histopathological grade II (3/4) and the overall survival at 1–3 years. In contrast, the overexpression for both miRNAs was seen in 2/10 patients, independent of tumor histopathological grade, with the overall survival at 5–6 years. According to the literature, miR-26a and miR-181c suppressed
the expression of MTDH and MAP4K4 genes, respectively, inhibiting the tumor-promoting effects in tumors. Conclusion:
Our data appear to highlight the clinical evidence to use miRNAs as new prognosis biomarkers, allowing better stratification of patients. Studies are in progress to evaluate more patients and identify a molecular signature able to predict
TNBC prognosis.

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Published

2026-04-01

How to Cite

Siqueira, B. D. S., Camargos, B. N. C. de, Freitas, A. L. de, Salles, P. G. de O., Lucena, C. Ênio M. de, Braga, L. da C., & Lopes, L. M. S. (2026). MIR-26A AND MIR-181C PROFILE HIGHLIGHT AS POTENTIAL PROGNOSIS BIOMARKERS IN TRIPLENEGATIVE BREAST CANCER PATIENTS. Mastology, 32(suppl.2). https://doi.org/10.29289/259453942022V32S2020

Issue

Vol. 32 No. suppl.2 (2022)

Section

Commented Poster

License

Copyright (c) 2026 Bárbara Danielle Silva Siqueira, Bianca Nataliene Carvalho de Camargos, Ana Luiza de Freitas, Paulo Guilherme de Oliveira Salles, Clécio Ênio Murta de Lucena, Letícia da Conceição Braga, Luciana Maria Silva Lopes

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This work is licensed under a Creative Commons Attribution 4.0 International License.